Shrna depletion
Splet14. apr. 2024 · MPI depletion in primary FLT3 ITD AML samples causes ATF6 ... Representative plots of flow cytometry fluorescence intensity plot NT shRNA and MPI … SpletOur study aimed to investigate the effect of cIAP1 gene depletion on the chemosensitivity of ovarian cancer cells. We developed a novel short hairpin RNA (shRNA) plasmid …
Shrna depletion
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SpletSETDB1 depletion and selective H3K9me3 loss in compartment A After terminal differentiation, mouse bone marrow-derived macrophages were infected with purified lentiviruses expressing two nontargeting (NT) shRNAs or two distinct shRNAs specific for SETDB1, causing a depletion of RNA and protein >90% ( Supplemental Fig. S1A ). SpletUBE2D3 depletion changed the global proteome, with the levels of proteins from metabolic pathways, in particular retinol metabolism, being the most affected. However, the impact of UBE2D3 depletion on the ubiquitinome was much more prominent. Interestingly, molecular pathways related to mRNA translation were the most affected.
Splet29. mar. 2024 · As shown in Figure 2H, the shRNA-mediated depletion of TASK-3 also suppresses the pH-dependent potassium current in MCF-10F cells. These data clearly show that TASK-3 is a functional channel in both cell lines, and that the pH-dependent portion of the macroscopic potassium current is carried through TASK-3 channels. SpletDEMETER2 (D2), as with DEMETER (D1), seeks to explain the observed shRNA depletion in each sample as a combination of gene knockdown effects and off-target seed effects. …
Splet10. apr. 2024 · ME2-depletion significantly reduced the migration of SK-Hep 1 cells (Figure 1B). In contrast, overexpression of ME2 enhanced cell migration ( Figure 1 C). Consistent with the results in SK-Hep 1 cells, ME2-depletion also inhibited cell migration in human hepatocellular carcinoma Huh 7 cells ( Figure 1 D,E), whereas up-regulation of ME2 … Splet19. sep. 2024 · Using a comprehensive dataset of 501 cell lines screened with shRNA on a genome-scale level, the CTD 2 team found that shRNA depletion scores for pairs of …
Splet28. avg. 2015 · A random, short-hairpin-RNA (shRNA)-expressing library was screened in primary FRDA fibroblasts and identified two shRNAs that reverse the growth/viability defect in an in vitro m
SpletPossemato et al. detailed an in vivo pooled shRNA screening approach to identify that the serine synthesis pathway is essential in breast cancer 1. A list of 2,752 genes gathered … hartmans plantsSplet01. apr. 2024 · Here, we show that the colorectal cancer cell line SK-CO-1 lacks detectable ATM protein expression and is sensitive to the PARP inhibitor olaparib. Similarly, HCT116 … hartman sorrento fire pitSplet30. sep. 2014 · The proliferation effect of each shRNA in each cell line was assessed by transducing a population of 11M cells with one shRNA-virus per cell and determining the relative enrichment or depletion of ... hartman takedownThe significant and preferential depletion of shRNAs causing knockdown of proteins known to be essential for cell viability during Chk1i-induced replication stress (RRM1, RRM2, and RPA2), PARP1/2 ... hartmans septicSplet12. apr. 2024 · Depletion of HIGD2A induced mitochondrial stress and attenuated the ATP generation capacity of HCC cells Generally, the process of cell proliferation requires high … hartman steamer turn table trunkSplet07. jun. 2024 · 新型混合shRNA筛选文库2014震撼问世：shRNAmir. 高通量干扰研究对shRNA提出了更高的要求，即单拷贝shRNA载体整合到基因组上也要有干扰潜能。这才 … hartman sorrento coversSpletLeading a cross-divisional project team of 11 people, deploying accelerometry devices within trials. Leveraging terabyte-scale clinical data to monitor physical activity and health. Research... hartman teak protector